Testing the efficiency of SIGS-mediated RNAi against M. Persicae

3 experiments were conducted and methods are written up. Experiments conducted were to assess whether spraying dsRNA can silence a target gene and affect aphid movement and reproduction.

Experiment 1: aphids sprayed while on plants.

Experiment 2: aphids feeding on sprayed plants.

Experiment 3: aphids alone were sprayed and then transferred to the plant to feed for 14 days. One aphid life cycle is completed in 14 days, so reproduction was assessed after 14 day period.

Nanoparticles were bound to dsRNA to see if it would improve the delivery to the plants and aphids, and improve the efficacy of treatments.

To summarise the results, there was a stronger effect on movement than reproduction in all tested conditions. No improved effect observed with nanoparticle-bound dsRNA.

I have attached a draft (which contains all sections) with feedback to help, a lot may seem repetitive. Please re-write concisely and professionally. Need help with statistical analysis, may need to be re-done (Fisher’s exact/chi squared test when comparing number of aphids for reproduction or number of dead aphids etc. highlighted in the document). Please do stats analysis where required even if I have not analysed.

Please note that I’ve attached an excel file with all the data and one-way ANOVA and t-test results. Post-hoc Tukey’s test results were done on an online platform, not present here, might need to be re-done.

Graphs already done. I will edit that myself, please use that for reference when writing results. Some graphs may present the same results in different ways (e.g. 4.11 and 4.12). However, please note comment on figure 4.10. Wouldn’t mind some guidance/ideas with compiling graphs 4.8 to 4.10 together.

I will be requesting an additional chapter (final discussion for the whole of the thesis) to be completed after this order has been done, would like for the same writer to work on it, thank you.

Thank you so much.

UPD: Cl will place order for another chapter which is related to that paper tomorrow. But it can be in progress when this order is completed. So, the same wr should be assigned and next order should be put on hold until this one is completed.

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Lab Report 1 Essay

this is a lab report which contains three 2 previous assignments that we have already done ( II will upload these 2 assignments) and for the new one, I will provide everything that you need for this lab report. There is 3 lab report and I need to combine them into 1 lab report.
There is a link that I want you to use as one of the references. So to make sure so there is module 1 part 1, 2, and 3. I already have 1 and 2 I need part 3 which will include 1, 2, and 3.

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I need your confirmation for your help regarding my Phd thesis. It is an experiment comparing the RNA seq

I need your confirmation for your help regarding my Phd thesis. It is an experiment comparing the RNA seq of two treatments. The first for growth of Dunaliella cells in inorganic autotrophic medium, the second is the same cells growing in mixture of organic and inorganic mixotrophic medium. The RNA seq data are already analyzed, and I have the resulting heat map and Gene ontologies and a combined transcriiptome assembly for the used 6 replicates (3 replicates for each treatment of the 2 treatments). I have no experience to guide with detailed instructions. I needed abstract, introduction, materials and methods, results, and discussion for the thesis. It needs no software experience but I think you may need use user friendly bioinformatics websites to help you to find interpretations and detect the pathways that are up or down regulated in response to the different conditions of growth for the cells. I also may need to understand some information that you will provide me on your written manuscriipt as lt is my first RNAseq experiment.

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completing Assignment: Take a moment to list your past experience(s), observation(s) of safety violation, or near-miss accident in the laboratory that was due to unsafe infrastructure, experiment design or equipment operation. Using the table format, propose one or more specific solutions for avoiding such a problem to re-occur in the laboratory.

Instructions for completing Assignment: Take a moment to list your past experience(s), observation(s) of safety violation, or near-miss accident in the laboratory that was due to unsafe infrastructure, experiment design or equipment operation. Using the table format, propose one or more specific solutions for avoiding such a problem to re-occur in the laboratory.

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a 2.5 research proposal paper about “how environmental pollution affects pregnancy.” Talk about low birth rate and the correlation of low birth weights and birth problems due to pollution. You need to establish a hypothesis and how you would go about changing the norm related to your paper or studying differently to fix a problem. I have attached a document which details different sections of a research paper and what you can add to each section to make it relevant to your cause. Make sure to refer to the two samples that I have provided for your information.

PLEASE do not plagiarize. I have had an issue with a tutor before due to plagiarism. I need this paper by tomorrow thursday May 12 at 6pm EST. Paper must be 2.5 pages and single spaced. I have two more papers as an example that I need to send to you since it must follow the same way according to the professor. Please let me know how can I send them to you.

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a 2.5 research proposal paper about “how environmental pollution affects pregnancy.” Talk about low birth rate and the correlation of low birth weights and birth problems due to pollution. You need to establish a hypothesis and how you would go about changing the norm related to your paper or studying differently to fix a problem. I have attached a document which details different sections of a research paper and what you can add to each section to make it relevant to your cause. Make sure to refer to the two samples that I have provided for your information.

PLEASE do not plagiarize. I have had an issue with a tutor before due to plagiarism. I need this paper by tomorrow May 12 at 4pm EST. Paper must be 2.5 pages and single spaced. I have two more papers as an example that I need to send to you since it must follow the same way according to the professor. Please let me know how can I send them to you.

Place this order or similar order and get an amazing discount. USE Discount code “GET20” for 20% discount

a 2.5 research proposal paper about “how environmental pollution affects pregnancy.” Talk about low birth rate and the correlation of low birth weights and birth problems due to pollution. You need to establish a hypothesis and how you would go about changing the norm related to your paper or studying differently to fix a problem. I have attached a document which details different sections of a research paper and what you can add to each section to make it relevant to your cause. Make sure to refer to the two samples that I have provided for your information.

PLEASE do not plagiarize. I have had an issue with a tutor before due to plagiarism. I need this paper by tomorrow May 12 at 4pm EST. Paper must be 2.5 pages and single spaced. I have two more papers as an example that I need to send to you since it must follow the same way according to the professor. Please let me know how can I send them to you.

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For this article “Module-2-DQ paper-Sakamoto et al._2018_Enzyme‑linked immunosorbent assay for the quantitative-qualitative analysis of plant secondary metabolites. Write a summary of the above article in one paragraph. Following are the criteria that students need to follow to write it: The paragraph should be within the minimum of 400. The paragraph’s content briefly represents all sections of the paper but highlights the significant findings. The write-up should be in your language. You cannot copy any sentences from the paper or other resources but can paraphrase to avoid plagiarism. Your submission should reflect that you have clearly understood and written it in a coherent form and professional language. If you wish to add other relevant ideas besides the assigned paper (optional), you can include references listed at the paragraph’s bottom. See upload 1

2. This assignment aims to assess the students’ ability to find scholarly resources in the area of Immunology. Each student must find a peer-reviewed research paper published anytime between 2016 to 2022. We are looking for a recently published article. You can use one of the following topics to narrow down your search; Innate immune system Adaptive immune system Antigen and Aantibody reactions to diagnose the diseases. Immune assay Cytokines Autoimmune disorders Hypersensitivity reactions Immunodeficiency Disorders Immunization to Infectious Diseases Find a research paper that usually contains an Introduction, Research question/s, Methods (experiments to generate data), Results

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health problem to explore in detail by using a descriptive epidemiologic approach. For examples of health problems or diseases that might be studied, refer to list below or visit the website shown. Diseases or health problems include but not limited to HIV, Gonorrhea, Listeriosis, Hepatitis A, B or C, Legionellosis, Lyme Disease, Dengue, Botulism, West Nile, Infectious Diseases, Anthrax, Mumps, SARS-CoV, Ebola, etc. https://www.cdc.gov/mmwr/volumes/64/wr/mm6453a1.htm?s_cid=mm6453a1_w The objectives of this exercise are as follows: • To gain experience in describing and analyzing the distribution of a health disorder in a population • To become familiar with various sources of data for the epidemiologic description of a health disorder • To enhance the ability to make sound epidemiologic judgments related to public health problems

Model for organization of paper: 1. Define the problem (nature, extent, significance, etc.). 2. Describe the agent. 3. Describe the condition (briefly). 4. Examine the above sources for data on morbidity and mortality in the selected health problem. 5. Summarize these data on the distribution of the selected health problem according to the following factors, using tables, graphs, or other illustrations whenever possible. A. Host characteristics 1. Age 2. Sex 3. Nativity 4. Marital status 5. Ethnic group B. Environmental attributes 1. Geographic areas 2. Social and economic factors 1. Income 2. Housing 3. Occupation 4. Education C. Temporal variation 1. Secular 2. Cyclic 3. Seasonal 4. Epidemic D. Any additional characteristic that contributes to an epidemiologic description of the disease 6. Summarize any current hypotheses that have been proposed to explain the observed distribution. 7. List the principal gaps in knowledge about the distribution of the health problem. The paper should be 10 double-spaced pages (excluding references, tables, and figures): Papers (APA Format) MUST include: Outline In-text citations Work cited page Papers (APA Format) CAN include: Pictures Graphs Charts

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Writer’s Choice Essay

This will be a lab report/scientific paper.

Its BIOINFORMATICS CLASS MASTER LEVEL!

You need to focus on paper on the sequence analysis and bioinformatics and not so much on literature. The project is to use the tools you learned earlier in the semester. Organize your results in this way: First, you and I obtained the sequence from NCBI. Then we observed the conserved domains using the CDD. I suggested you also do this for Pfam. Discuss the different domains observed and any active sites or binding sites. That’s three of the five approaches required.Please if you could use ClinVar or OMIM, they would count as approaches. OMIM will also give you some general information about these genes and phenotypes, so I think that would be a good resource for you to use. Remember you need to focus on BRAC 1 and BRAC 2. Ask question if you dont understand.

Please read “Paper” and add staff I wrote earlier.

https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi?INPUT_TYPE=precalc

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